midi macs separator Search Results


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Miltenyi Biotec midi macs ls separation columns
Midi Macs Ls Separation Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec midi macs separator
Midi Macs Separator, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec macs® midi magnetic separation column
Macs® Midi Magnetic Separation Column, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec midi macs cd34 progenitor cell isolation kit
Midi Macs Cd34 Progenitor Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd34 microbead kit
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Cd34 Microbead Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec midi macs cd4 t cells magnetic isolation kit
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Midi Macs Cd4 T Cells Magnetic Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd90 2 microbeads
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Cd90 2 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Illumina Inc nextseq 500 550 v2 kits
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Nextseq 500 550 V2 Kits, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec midi mac separation column
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Midi Mac Separation Column, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Bio-Rad nitrocellulose membranes bio rad laboratories cat
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Nitrocellulose Membranes Bio Rad Laboratories Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec midi macs separator unit according to the manufacturer's instructions
( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of <t>CD34+</t> cells transduced with shTP53 is significantly lower than in <t>CD34+</t> cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Midi Macs Separator Unit According To The Manufacturer's Instructions, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of CD34+ cells transduced with shTP53 is significantly lower than in CD34+ cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Journal: Oncotarget

Article Title: Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model

doi: 10.18632/oncotarget.25581

Figure Lengend Snippet: ( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of CD34+ cells transduced with shTP53 is significantly lower than in CD34+ cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The CD34+ cell fraction was then isolated from the MNCs using the Midi MACS system (CD34+ Microbead Kit; Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer's instructions.

Techniques: Transduction, Expressing, Comparison

( A ) CFU results of knockdown of TP53 by shRNA: knockdown of TP53 did not influence colony numbers of CD34+ cells as compared to shScr as a control. ( B ) CFU results of knockdown of cells transduced with wt TP53 or TP53 mutations. After irradiation there was a weaker reduction in cell colony numbers in CD34+ cells transduced with conformational mutations (R175H and R249S), whereas CD34+ cells transduced with contact mutations (R248W and R273H) did not differ from those transduced with wt TP53. ( C ) Erythropoiesis in CD34+ cells overexpressing wtTP53 or expressing the contact mutations R248W and R273H was significantly reduced as compared to CD34+ cells transduced with conformational mutations. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Journal: Oncotarget

Article Title: Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model

doi: 10.18632/oncotarget.25581

Figure Lengend Snippet: ( A ) CFU results of knockdown of TP53 by shRNA: knockdown of TP53 did not influence colony numbers of CD34+ cells as compared to shScr as a control. ( B ) CFU results of knockdown of cells transduced with wt TP53 or TP53 mutations. After irradiation there was a weaker reduction in cell colony numbers in CD34+ cells transduced with conformational mutations (R175H and R249S), whereas CD34+ cells transduced with contact mutations (R248W and R273H) did not differ from those transduced with wt TP53. ( C ) Erythropoiesis in CD34+ cells overexpressing wtTP53 or expressing the contact mutations R248W and R273H was significantly reduced as compared to CD34+ cells transduced with conformational mutations. ( * P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: The CD34+ cell fraction was then isolated from the MNCs using the Midi MACS system (CD34+ Microbead Kit; Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer's instructions.

Techniques: Knockdown, shRNA, Control, Transduction, Irradiation, Expressing