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Image Search Results
Journal: Oncotarget
Article Title: Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model
doi: 10.18632/oncotarget.25581
Figure Lengend Snippet: ( A ) Overview of the workflow. ( B ) Under stress conditions apoptosis rate of CD34+ cells transduced with shTP53 is significantly lower than in CD34+ cells transduced with shScr or in non-transduced cells. ( C ) Under stress conditions CD34+ cells over-expressing wtTP53 have significantly higher apoptosis rates in comparison to controls and to cells with TP53 mutations R175H, R248W and R249S. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Article Snippet: The CD34+ cell fraction was then isolated from the MNCs using the Midi MACS system (
Techniques: Transduction, Expressing, Comparison
Journal: Oncotarget
Article Title: Effect of TP53 contact and conformational mutations on cell survival and erythropoiesis of human hematopoietic stem cells in a long term culture model
doi: 10.18632/oncotarget.25581
Figure Lengend Snippet: ( A ) CFU results of knockdown of TP53 by shRNA: knockdown of TP53 did not influence colony numbers of CD34+ cells as compared to shScr as a control. ( B ) CFU results of knockdown of cells transduced with wt TP53 or TP53 mutations. After irradiation there was a weaker reduction in cell colony numbers in CD34+ cells transduced with conformational mutations (R175H and R249S), whereas CD34+ cells transduced with contact mutations (R248W and R273H) did not differ from those transduced with wt TP53. ( C ) Erythropoiesis in CD34+ cells overexpressing wtTP53 or expressing the contact mutations R248W and R273H was significantly reduced as compared to CD34+ cells transduced with conformational mutations. ( * P < 0.05; ** P < 0.01; *** P < 0.001).
Article Snippet: The CD34+ cell fraction was then isolated from the MNCs using the Midi MACS system (
Techniques: Knockdown, shRNA, Control, Transduction, Irradiation, Expressing